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reporter plasmid pg5-luc containing five gal4 dna binding sites cloned upstream of a minimal promoter element and the firefly luciferase gene  (Promega)

 
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    Structured Review

    Promega reporter plasmid pg5-luc containing five gal4 dna binding sites cloned upstream of a minimal promoter element and the firefly luciferase gene
    Reporter Plasmid Pg5 Luc Containing Five Gal4 Dna Binding Sites Cloned Upstream Of A Minimal Promoter Element And The Firefly Luciferase Gene, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/firefly+luciferase+reporter+plasmid+pg5+luc/gal4+responsive+luciferase+reporter/pmc03287979-275-18-24
    Average 90 stars, based on 1 article reviews
    reporter plasmid pg5-luc containing five gal4 dna binding sites cloned upstream of a minimal promoter element and the firefly luciferase gene - by Bioz Stars, 2026-10
    90/100 stars

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    Related Articles

    Luciferase:

    Article Title: SP1/TGF-β1/SMAD2 pathway is involved in angiogenesis during osteogenesis
    Article Snippet: Endothelial tube formation was analyzed using a Leica DMI6000B light and fluorescence microscope (Leica Microsystems, Inc.) using a green filter (magnification ×100). .. The firefly luciferase reporter plasmid (pG5 luc) and constitutively active Renilla luciferase control plasmid (pRL- Renilla ) were purchased from Promega Corporation. ..

    Article Title: SP1/TGF‑β1/SMAD2 pathway is involved in angiogenesis during osteogenesis.
    Article Snippet: .. The firefly luciferase reporter plasmid (pG5 luc) and constitutively active Renilla luciferase control plasmid (prl-Renilla) were purchased from Promega corporation. ..

    Plasmid Preparation:

    Article Title: SP1/TGF-β1/SMAD2 pathway is involved in angiogenesis during osteogenesis
    Article Snippet: Endothelial tube formation was analyzed using a Leica DMI6000B light and fluorescence microscope (Leica Microsystems, Inc.) using a green filter (magnification ×100). .. The firefly luciferase reporter plasmid (pG5 luc) and constitutively active Renilla luciferase control plasmid (pRL- Renilla ) were purchased from Promega Corporation. ..

    Article Title: SP1/TGF‑β1/SMAD2 pathway is involved in angiogenesis during osteogenesis.
    Article Snippet: .. The firefly luciferase reporter plasmid (pG5 luc) and constitutively active Renilla luciferase control plasmid (prl-Renilla) were purchased from Promega corporation. ..

    Control:

    Article Title: SP1/TGF-β1/SMAD2 pathway is involved in angiogenesis during osteogenesis
    Article Snippet: Endothelial tube formation was analyzed using a Leica DMI6000B light and fluorescence microscope (Leica Microsystems, Inc.) using a green filter (magnification ×100). .. The firefly luciferase reporter plasmid (pG5 luc) and constitutively active Renilla luciferase control plasmid (pRL- Renilla ) were purchased from Promega Corporation. ..

    Article Title: SP1/TGF‑β1/SMAD2 pathway is involved in angiogenesis during osteogenesis.
    Article Snippet: .. The firefly luciferase reporter plasmid (pG5 luc) and constitutively active Renilla luciferase control plasmid (prl-Renilla) were purchased from Promega corporation. ..



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    A plasmid mixture of pG5, TK-renilla reporter constructs, and pBIND-GAL4-TTF-1 were transfected into A549 cells. After transfection for 6 h, the A549 cells were further cultured with the indicated dosage of leptin for 24 h. The cells were lysed and luciferase activity was measured and normalized by renilla luciferase activity. Results are shown as fold induction in relation to the activity in controls. * P <0.01 vs the control cells ( n = 3).

    Journal: PLoS ONE

    Article Title: Leptin Promotes Fetal Lung Maturity and Upregulates SP-A Expression in Pulmonary Alveoli Type-II Epithelial Cells Involving TTF-1 Activation

    doi: 10.1371/journal.pone.0069297

    Figure Lengend Snippet: A plasmid mixture of pG5, TK-renilla reporter constructs, and pBIND-GAL4-TTF-1 were transfected into A549 cells. After transfection for 6 h, the A549 cells were further cultured with the indicated dosage of leptin for 24 h. The cells were lysed and luciferase activity was measured and normalized by renilla luciferase activity. Results are shown as fold induction in relation to the activity in controls. * P <0.01 vs the control cells ( n = 3).

    Article Snippet: The pG5 luc firefly luciferase reporter plasmid containing the five GAL4-binding sites upstream of the TATA box was obtained from Promega.

    Techniques: Plasmid Preparation, Construct, Transfection, Cell Culture, Luciferase, Activity Assay, Control